Learn how to Edit Bacteria with CRISPR
$59.00 - $129.00
This kit comes with an example experiment that teaches you many molecular biology and gene engineering techniques.
However, if you are looking to use this kit to get a deeper understanding of bioengineering we recommend that you purchase our Learn Bioengineering Bundle which includes this kit as part of it.
We Fulfill and Ship in 2-3 days in the US. Overnight is not required and neither are ice packs.
Want to really know what this whole CRISPR thing is about? Why it could revolutionize genetic engineering? This kit includes everything you need to make precision genome edits in bacteria at home including Cas9, gRNA and Template DNA template for an example experiment.
Includes example experiment to make a genome mutation(K43T) to the rpsL gene changing the 43rd amino acid, a Lysine(K) to a Threonine(T) thereby allowing the bacteria to survive on Strep media which would normal prevent its growth.
Kit contains everything you need for around 5 experiments or more
- LB Agar
- LB Strep/Kan/Arabinose Agar
- Glass bottle for pouring plates
- Non-pathogenic E. coli DH5a bacteria
- Inoculation Loops
- 10-100uL variable volume adjustable pipette(1uL increments)
- Box of 96 Pipette Tips
- 14 Petri Plates
- Microcentrifuge tube rack
- Nitrile Gloves
- Microcentrifuge tubes
- 50mL Tube for measuring
- Bacterial transformation buffer 25mM CalCl2, 20% PEG 8000
- LB Media for transformation recovery
- Cas9 plasmid
- gRNA plasmid
- Template DNA
**Perishables included in this kit have been Freeze Dried. They will be viable up to a month at Room temperature**
Some items in this kit need to be stored in a fridge and a freezer upon you receiving them.
Take a look at our Experiment Protocol
Access plasmid maps.
Ordering the Refill/Classroom kit? Here's what it includes:
- Cas9 plasmid
- gRNA plasmid
- Template DNA
- Strep/Kan/Arabinose Agar Media
- LB Agar Media
- LB Media
- 20 microcentrifuge tubes
- 14 plates
- Non-pathogenic DH5a E. coli bacteria
- Inoculation loops
- Bacterial Transformation Buffer
**Perishables included in this kit have been Freeze Dried. They will be viable up to a month at Room temperature**
*The REFILL KIT kit does not contain a pipette, pipette tips and a glass bottle for making media. These must be user supplied.
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Out of stock
Reviews
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Just what the doctor ordered
Great product
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Great introduction to Cas9 DNA editing.
Just finished growing my own DNA edited bacteria in my home office. I come from a microelectronics background so working with precision tools and small amounts of material were natural. Succeeding the first time working with engineering DNA was a true rush. This was a lot of science fun and I can't wait to try another kit from Odin.
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Great product!
The kit was easy to use and a great way to get experience with genetic engineering. There were just a few times where the directions could have a bit more detail, however, they were still sufficient and generally very clear.
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Contains absolutely everything
The kit is very complete, which is useful for those with no other lab equipment or resources. It is good to see that a perishables replacement kit is now available. I would also like to have the protocols available electronically as a PDF.
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Wonderful introduction to CRISPR
As a retired professional in a different science field and having little or no micro/biology training I learned quite a bit with this kit and online tutorials. The folks, especially Peter at "The Odin" were very helpful and professional responding to all my emails with detailed explanations and guidance. I strongly recommend this kit for folks who would like to learn and explore some broad understanding of this astounding technology.
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Great Product
Great Product! A really fun experiment and great introduction to the technology. I would recommend this product to anyone wanting to learn the basics or anyone that once to be a scientist for a weekend.
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Unique Product
Good product to get hands in genetic engineering. Fast shipping
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PLEASE MAKE REPLACEMENT KIT!!!
Great kit, worked well! Please consider making a cheaper replacement kit with just the bacteria, tranformation mix, LB agar, LB Strep/Kan/Ara Agar, LB media, Cas9 plasmid, gRNA, and template DNA. Many of us who want to repeat this experiment in the classroom yearly already have the other materials in our labs and need to watch our budgets. I will buy again!
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Exactly as promised.
I received the kit very quickly, within the week I think. Everything was as promised and support was good as well. It worked perfectly and the instructions were helpful and easy to follow. I would definitely recommend ordering from them and will use The Odin for future classroom supply needs.