E. coli DH5a

E. coli DH5a strain freeze dried

Strain for DNA replication when cloning plasmids in bacteria.

For creating competent cells see here: http://openwetware.org/wiki/Preparing_chemically_competent_cells

Genotype

F^– endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(r_K^–m_K⁺), λ^–

• A Hoffman-Berling 1100 strain derivative (Meselson68)
• Promega also lists phoA
• Thiamine, arginine auxotroph
• Lactose nonutilizing
• Nalidixic acid resistant
• Recombination-deficient
• Relaxed phenotype
• Endonuclease A deficient
• Constitutive deoxyribose synthesis, good for cloning large plasmids.
• Amber suppressor UAG>CAG(Gln).
• Slow growth in M9 medium due to purB-20
• References:
• FOCUS (1986) 8:2, 9.
• Hanahan, D. (1985) in DNA Cloning: A Practical Approach (Glover, D.M., ed.), Vol. 1, p. 109, IRL Press, McLean, Virginia.
• Grant, S.G.N. et al. (1990) Proc. Natl. Acad. Sci. USA 87: 4645-4649 PMID 2162051.
• Meselson M. and Yuan R. (1968) Nature 217:1110 PMID 4868368.

**Perishables included in this kit have been Freeze Dried. They will be viable over a month at room temperature**