CRISPR Bacteria Gene Editing Kit

CRISPR Bacteria Gene Editing Kit

$129.00 USD

Intermediate

Cold Storage Required

|30 reviews

CRISPR Bacteria Gene Editing Kit

Use the same gene-editing tool behind Nobel Prize-winning research to rewrite living DNA - and read the results on an agar plate.

$129.00 USD
Additional Information

Width: 30.48 (cm)
Height: 17.78 (cm)
Depth: 30.48 (cm)

CRISPR Bacteria Gene Editing Kit
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Product Details

CRISPR-Cas9 is a programmable gene-editing system used throughout modern biology. In this kit, you’ll use it in E. coli to target a specific gene and introduce a defined change.

You’ll introduce the CRISPR components, direct Cas9 to the target, and use antibiotic selection to identify bacteria in which the edit worked.

Who Is It For

A good next step if you want to understand how CRISPR actually works by running the experiment yourself.

No prior lab experience is required to get started.

Students Students
Biohackers Biohackers
Science educators Science educators
Hands-on CRISPR experience Hands-on CRISPR experience

What's Included

Lab Supplies

Petri plates Petri plates
Adjustable micropipette, 10-100 µL Adjustable micropipette, 10-100 µL
Pipette tips Pipette tips
Microcentrifuge tubes Microcentrifuge tubes
Tube rack Tube rack
Sterile inoculation loops Sterile inoculation loops
Nitrile gloves Nitrile gloves
Agar preparation bottle Agar preparation bottle

Biological Materials

Non-pathogenic E. coli DH5a strain, freeze dried
Non-pathogenic E. coli DH5a strain, freeze dried
Cas9 expression plasmid
Cas9 expression plasmid
Guide RNA plasmid
Guide RNA plasmid
DNA repair template
DNA repair template

Media & Reagents

LB agar media
LB agar media
Selective antibiotic agar media (LB Strep/Kan/Arabinose)
Selective antibiotic agar media (LB Strep/Kan/Arabinose)
Transformation buffer (25mM CalCl2, 20% PEG 8000)
Transformation buffer (25mM CalCl2, 20% PEG 8000)
LB recovery media
LB recovery media

Shipping & Storage 

All biological materials are freeze-dried and remain stable at room temperature for up to one month, so ice packs or overnight delivery are not required.

Upon arrival, please place designated reagents in the fridge or freezer.

Orders are fulfilled and shipped within 2–3 days in the US.

Includes step-by-step experiment guide, CRISPR theory booklet, and plasmid maps.

What You Will Do

Transform bacteria with Cas9, guide RNA, and a DNA repair template
Target a specific genomic location using guide RNA
Grow edited and unedited bacteria side by side on antibiotic plates
Confirm successful edits by comparing colony survival rates

What You Will Learn

These are the same fundamental skills that show up throughout molecular biology and biotechnology labs.

CRISPR-Cas9 Mechanism

How CRISPR-Cas9 finds and cuts specific DNA sequences.

DNA Break Repair

How cells repair DNA breaks - and how that repair can be guided.

Plasmid Delivery

The role of plasmids in delivering genetic tools into cells.

Antibiotic Selection

Why antibiotic selection is a cornerstone of genetic engineering.

Step-by-Step Protocols

Every kit includes clear, easy-to-follow protocols that guide you through each experiment from start to finish.

Follow the steps at your own pace, and use the notes along the way to check your progress and troubleshoot.

B
Braylon W.
Classroom refill kit

The refill kit came with exactly what I needed in order to reuse the original kit. Clear instructions and over a great kit.

J
Joseph M.
Good and does what I need it to do

For what I'm doing which is genetically modifying plant DNA for taste and longevity its a good starter kit.

I'm not ready to fully go beyond a controlled environment just yet as it'll take me a bit to learn everything its good place to start with.

Still learning how it works just to use it safely though such that environmental damage does not occur.

l
loide l.m.d.f.
excellent

I haven't receivend the kit yet, but I'm sure it will meet all my expectations. Thank you!

A
AGNES Q.
Smooth and Straight-forward

AMAZING KIT! I got it super quickly. Inside there are Different elements to play with and explanation guide that teaches you how to work with CRISPR even-tough you have never used it before. I am having so much fun experimenting and exploring genome engineering!

A
Anonymous
best

Vraiment top qualité jadore

d
daniel t.
Great Intro to Technique

We followed the instructions very carefully and got textbook results on the first go. We took the additional step of trying to grow the naive bug on strep agar (negative results). This kit is a great accompaniment to study of CRISPR. A concise summary of the processes needed to assemble this kit might give students a better feel for its elegance (how the template DNA is produced, for example--a lot of work is needed to put a student in a position to do this kit!). That is not a complaint. This is a really great and enlightening product.

2
213
Tech in the peoples hands

This is an amazing intro to science most would feel out of reach. The quality of materials is better than I had expected. Highly recommended

R
Robert P.
CRISPR in the 21st century

The kit contains everything one needs to CRISPR some e. coli. If adequate pre-lab is done, it is quite understandable to most AP Bio students. Although it looks like a bacterial transformation that they have all done previously, they can understand what the Cas9 and the guide RNA is doing. I do have one recommendation - virtually all the kits I buy are for 8 stations/groups - this one is for 7. Drop some of the easily obtained or nice but unnessary things like gloves, 50 ml measuring device, and the microfuge tube rack, and give me enough of the nucleic acids, media, and bacteria to serve 8 stations.

I
Ian C.
amazing value

The kit includes everything you need, with an easy to follow protocol for learning CRISPr. The price speaks for itself. 10/10

A
Amanda L.
Very cool

Directions are a bit vague - yet I still succeed with zero
experience!
Highly recommend, very cool this is offered to the public.

A
Antonio C.
Gift for my GF

This is a gift for my GF, she study for become a biotechnologist and she is very happy when recive this kit.
Tnx for all ODIN

Sorry for my English but I come from Italy:)

A
Aleksandra
A fantastic product with the UK shipping

The Odin CRISPR/Cas9 bacterial kit is a creative and effective way for everyone who wants to learn biomedical engineering or biohacking. It has everything you need to perform a simple experiment and learn how to use basic laboratory equipment. Also, instructions are written in a very clear and engaging way and it has been shipped to the UK with no issues! Thanks the Odin!

M
Matthew B.
Just what the doctor ordered

Great product

P
Prof. T.
Great introduction to Cas9 DNA editing.

Just finished growing my own DNA edited bacteria in my home office. I come from a microelectronics background so working with precision tools and small amounts of material were natural. Succeeding the first time working with engineering DNA was a true rush. This was a lot of science fun and I can't wait to try another kit from Odin.

A
Anonymous
Great product!

The kit was easy to use and a great way to get experience with genetic engineering. There were just a few times where the directions could have a bit more detail, however, they were still sufficient and generally very clear.

A
Anonymous
Contains absolutely everything

The kit is very complete, which is useful for those with no other lab equipment or resources. It is good to see that a perishables replacement kit is now available. I would also like to have the protocols available electronically as a PDF.

M
Michael W.
Wonderful introduction to CRISPR

As a retired professional in a different science field and having little or no micro/biology training I learned quite a bit with this kit and online tutorials. The folks, especially Peter at "The Odin" were very helpful and professional responding to all my emails with detailed explanations and guidance. I strongly recommend this kit for folks who would like to learn and explore some broad understanding of this astounding technology.

A
Anonymous
Great Product

Great Product! A really fun experiment and great introduction to the technology. I would recommend this product to anyone wanting to learn the basics or anyone that once to be a scientist for a weekend.

M
Martina F.
Unique Product

Good product to get hands in genetic engineering. Fast shipping

A
Anonymous
PLEASE MAKE REPLACEMENT KIT!!!

Great kit, worked well!
Please consider making a cheaper replacement kit with just the bacteria, tranformation mix, LB agar, LB Strep/Kan/Ara Agar, LB media, Cas9 plasmid, gRNA, and template DNA. Many of us who want to repeat this experiment in the classroom yearly already have the other materials in our labs and need to watch our budgets.

I will buy again!

C
Celesta B.
Exactly as promised.

I received the kit very quickly, within the week I think. Everything was as promised and support was good as well. It worked perfectly and the instructions were helpful and easy to follow. I would definitely recommend ordering from them and will use The Odin for future classroom supply needs.

G
George T.
Amazing!

The kit was easy and very interesting. The only issue was that it could have included more inoculation loops, although there is enough to carry out one experiment, I don't think that there is enough for two or more.

Y
YANG J.
Great Kit!

It Works!

C
Camille L.
Best CRISPR Kit on the market

I am a high school student who purchased this kit because I had a passion to learn more about biohacking at home, and be involved with genetic engineering independently. This kit was the perfect stepping stool to give me first hand info on CRISPR and the whole process is so easy to understand, everything you need is right there in the kit, just be careful NOT to freeze your E. coli like I did :(

A
Arthur
Excellent Introduction to technical details of CRISPR

Rand three trials so far and every one was successful.

S
Sarah
Works, but Protocol is Confusing

Shipping took a while, but the kit included all materials including two stickers with the ODIN logo. The experiment was a success - we got a lot of growth on the plates. The only frustration was the protocol was confusing and wordy. I ended up rewriting it so it was more straightforward without the protocols and walkthroughs in the middle of the handout. Otherwise, great kit! Ordered the refill to introduce to the class next term.

J
James P.
Bio Art

I was able to successfully complete this experiment. I am now working on sequencing the before and after bacteria and using the resulting data to drive one of my videos. Thank you Odin.

M
Mike R.
Satisfied Curious Newbie!

Fascinated by CRISPR, I wanted to learn about it. This Kit was impressive - thoughtful, well organized, fully equipped with extra supplies for mess ups, affordable and backed by technical information. Just what I wanted. Thanks so much!!

J
Jake
Best CRISPR kit on the market

Okay, it's also kind of the only CRISPR kit on the market right now. But it's nevertheless a wonderful and very simple kit.

M
Mary
It works!

I have read a lot about CRISPR, but I didn't know it could be this easy. The kit arrived with everything I needed and the instructions were thorough and easy to understand.

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