Fluorescent Yeast Genetic Engineering Kit

Fluorescent Yeast Genetic Engineering Kit

$179.00 USD

Advanced

Cold Storage Required

|2 reviews

Fluorescent Yeast Genetic Engineering Kit

In this kit, you'll introduce a GFP plasmid into yeast using the lithium acetate transformation method, grow colonies on selective agar, and observe fluorescent expression under blue light. The techniques you practice here map directly to what happens in advanced eukaryotic and mammalian cell work.

$179.00 USD
Additional Information

Width: 30.48 (cm)
Height: 25.40 (cm)
Depth: 15.24 (cm)

Fluorescent Yeast Genetic Engineering Kit
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Product Details

Engineer yeast - the organism behind bread, beer, and the world's first recombinant insulin - to produce glowing proteins. Your first step into eukaryotic genetic engineering.

Bacteria are a great starting point for genetic engineering. But yeast is where things get more interesting. Saccharomyces cerevisiae is a eukaryote - its cells are organized more like human cells than bacterial cells are. That's why scientists used it to pioneer recombinant insulin in the 1980s, and why it's now central to research on aging, cancer biology, and next-generation therapeutics.

Who Is It For

A next step after bacterial engineering if you want to work with a eukaryotic organism and learn a different transformation workflow.

It is ideal for biotech students covering eukaryotic cell biology, as well as anyone curious about how organisms like yeast are engineered to produce useful compounds at an industrial scale.

Next step after bacteria kits Next step after bacteria kits
Biotech students Biotech students
Eukaryotic cell biology Eukaryotic cell biology
Yeast genetic engineering Yeast genetic engineering
Advanced hands-on learning Advanced hands-on learning

What's Included

Lab Supplies

Agar preparation bottle Agar preparation bottle
Adjustable micropipette (10–100 µL) Adjustable micropipette (10–100 µL)
Pipette tips Pipette tips
Sterile inoculation loops Sterile inoculation loops
Petri plates Petri plates
Tube rack Tube rack
50mL test tube for measuring 50mL test tube for measuring
Microcentrifuge tubes Microcentrifuge tubes
Nitrile gloves Nitrile gloves
Orange UV filter sheet  + Blue LED light Orange UV filter sheet + Blue LED light

Biological Materials

Saccharomyces cerevisiae yeast strain
Saccharomyces cerevisiae yeast strain
Preengineered strain of yeast containing GFP plasmid, freeze dried
Preengineered strain of yeast containing GFP plasmid, freeze dried
Yeast GFP expression plasmid (100ng/uL)
Yeast GFP expression plasmid (100ng/uL)

Media & Reagents

YPD agar
YPD agar
Selective YPD agar (G418)
Selective YPD agar (G418)
Transformation buffer (0% PEG 8000, 200mM LiAc, 0.1mg/mL salmon sperm DNA)
Transformation buffer (0% PEG 8000, 200mM LiAc, 0.1mg/mL salmon sperm DNA)
YPD broth
YPD broth
Sterile water
Sterile water

Shipping & Storage 

Some items require refrigeration and freezer storage upon arrival.

Includes yeast transformation protocol, guide to yeast genetics, calibrated micropipette, and 14 petri plates.

What You Will Do

Prepare yeast cells for transformation using the lithium acetate method
Introduce a GFP expression plasmid into live yeast cells
Plate on selective agar and grow transformed colonies
Visualize fluorescence using the included blue light and UV filter

What You Will Learn

These are the same fundamental skills that show up throughout molecular biology and biotechnology labs.

Eukaryotic Complexity

Why eukaryotic genetic engineering is more complex than bacterial work - and why that complexity matters.

Eukaryotic Gene Expression

How gene expression and protein production differ between eukaryotes and bacteria.

Yeast Transformation

Yeast transformation techniques used in academic and industrial labs.

Yeast Applications

Why yeast is the workhorse organism for pharmaceutical production and aging research.

Read Articles About 

The Fluorescent Yeast Genetic Engineering Kit

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Step-by-Step Protocols

Every kit includes clear, easy-to-follow protocols that guide you through each experiment from start to finish.

Follow the steps at your own pace, and use the notes along the way to check your progress and troubleshoot.

A
Aaron R.
5 stars

I’d be very appreciative if Odin started selling crispr kits for insects! I love the yeast and bacteria kits I’ve gotten so far, feel free to contact me if insect kits ever happen

J
James J.
Glowing Brew

Started as a demo for Intro to Biotech course and students made a project out of it to mass produce the protein and add to other food stuffs.

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